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KMID : 0613820120220091137
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Journal of Life Science
2012 Volume.22 No. 9 p.1137 ~ p.1144
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Eupatorium chinensis var. simplicifolium Root Extract Inhibits the Lipopolysaccharide-Induced Inflammatory Response in Raw 264.7 Macrophages by Inhibiting iNOS and COX-2 Expression
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Lee Jin-Ho
Kim Dae-Hyun
Shin Ji-Won
Park Sae-Jin
Kim Yoon-Suk
Shin Yu-Su
Yu Ji-Yeon
Kim Tack-Joong
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Abstract
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Inflammation is a host defense mechanism that is activated in response to harmful substances or pathogens. However, an excessive inflammatory response is a problem in itself. Macrophages secrete inflammatory mediators such as nitric oxide (NO) or cytokines through various pathways such as the nuclear factor kappa B (NF-κB)-activated pathway after recognizing pathogen-like lipopolysaccharides (LPSs). In this study, anti-inflammatory effects of Eupatorium chinensis var. simplicifolium (EUC) extracts were investigated using LPS-stimulated RAW 264.7 macrophages. The EUC root extract significantly reduced NO production, inducible nitric oxide synthase (iNOS) expression, and cyclooxygenase-2 expression in a concentration-dependent manner. In addition, the EUC root extract reduced phosphorylation of mitogen-activated protein kinases and protein kinase B, which is upstream of NF-κB. The EUC root extract also reduced the degradation of inhibitory kappa B. These results indicate that EUC root extract exerts anti-inflammatory effects, which are mediated by inhibition of iNOS expression and the NF-κB pathway.
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KEYWORD
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Eupatorium chinensis var. simplicifolium, cyclooxygenase-2, inflammation, nitric oxide, NF-κB pathway
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